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KMID : 0385020000160020087
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Korean Journal of Laboratory Animal Science
2000 Volume.16 No. 2 p.87 ~ p.92
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Immunological characteristics of the transgenic mice expressing a cytokine in the skin for the production of skin grafted animals
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김태윤/Kim, Tae Yoon
박영일/김경은/김명옥/이정웅/황하영/변승준/Bahk, Young Yil/Kim, Kyung Eun/Kim, Myoung Ok/Lee, Jeong Woong/Hwang, Ha Young/Byun, Sung June
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Abstract
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IL-10 inhibits macrophage-dependent antigen presentation, a variety of cytokine production, and generation of allospecific cells in vitro. These findings have lead to the widespread expectation that IL-10 may be a useful immunosupressive agent to inhibit allograft rejection or autoimmunity in vivo.
In the present study, to understand the immunological characteristics of the transgenic mice expressing a IL-10 in the skin, we have generated transgenic mice carrying mouse IL-10 gene under control of the human keratin 14 promotor. Most founder mice died between 2 to 3 weeks of age. Death may be associated with the excess of endogenous IL-10. To study the effect of the expression of cytokines mRNA in the skin of transgenic mice, a method of semiquantitation RT-PCR was performed. The transgenic mice showed the increased expression of INF-γ, IL-1α, IL-1β and TNF-α. The results indicate that IL-10 transgenic mice might be involved in the immune system via the cytokine network.
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KEYWORD
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